Charme Skin Care System
The Skin Care Solution
Acne Clinical Trial The following article appeared in the "Cosmetic Dermatology Journal" in February, 2004. It was a study to determine the efficacy and tolerability of electrolized oxidized water (EOW) produced by the Charme™ system in treating mild to moderate acne.
Alpesh Desai, DO; Cynthia J. Tam, DO; Kinnari Bhakta, BS; Tajas Desai, DO: Rashmi Sarkar, MD; Neil B. Desai. BS
Dr. Alpesh Desai is a Dermatologist and Clinical Researcher at Coast Dermatology in Torrance, California. Dr. Tarn is an intern at Philadelphia College of Osteopathic Medicine, Pennsylvania. Ms. Bhakta is a medical student and Dr. Tejas Desai is an intern at University of North Texas Health Science Center, Fort Worth. Dr. Sarkar is a Dermatologist at Safdarjung Hospital & Vardhman Mahavir Medical College, New Delhi, India. Mr. Desai is a student at Dartmouth College, Hanover, New Hampshire. Dr. Alpesh Desai is a consultant and advisory board member for Mikuni
Corporation. Drs. Tarn, Tejas Desai and Sarkar, and Ms. Bhakta and Mr. Desai report no actual or potential conflict of interest in relation to this article.
The purpose of this study was to determine the efficacy and the tolerability of the Charme™ system, a hand-held device that electrolyzes a water-based sodium chloride solution and delivers electrolyzed oxidized water (EOW) to the skin, in the treatment of mild to moderate acne. A total of 31 patients with mild to moderate acne vulgaris were enrolled in this 8-week, open-label, split-face pilot study. Acne lesion counts and physician global assessments were taken at each visit, as well as patient satisfaction and global assessments and high-resolution digital photographs. Statistical analysis of the results for treatment versus baseline scores was performed using a Hotelling T test and repeated-measures analysis of variance. Values of .04 or less were considered statistically significant. A total of 25 patients (mean age, 24.7 years, ranging from 15 to 47.years) completed the study. The mean reduction in total acne lesion counts from baseline to the end of treatment was 40.9 for the EOW-treated side (right side) and 18 for the untreated side (Heft side) (P=.04). Investigators observed a significant mean reduction in inflammatory lesions, both papules and pustules (P<01), as well as in the number of individual papules (75.7 vs 19.4) and pustules (42 vs 7.38) on the treated side versus the untreated side (P=.Q2). The mean reduction of the noninflammatory lesions, however, was not significant (P=.336). Physician global assessment indicated that 100 of the patients showed clinical improvement of 25 or more, while 50 of the patients observed a moderate improvement of 50 or more at the end of the treatment period. Interestingly. 75 of the patients preferred the EOW delivery system to other conventional treatment modalities. Only 5 patients had transient, cutaneous adverse effects, such as pruritus, erythema, burning/stinging, and/or peeling, that ceased by week 2. Patient satisfaction with the EOW system was rated high, with 80 of the patients in the study assessing the EOW device as good or excellent, while 16 rated the device as fair. Only 4 of the patients were dissatisfied with the device. The pilot study demonstrated that the EOW system is an effective and well-tolerated method of treatment for patients with acne. The device appeared to be effective primarily on inflammatory acne lesions. This is most likely attributed to the antimicrobial properties of the EOW that reduce the Propionibacterium acnes populations.
Antimicrobial Effects of Low pH Water in a Laboratory Setting
Acne vulgaris is an extremely common condition that affects more than 40 to 50 million individuals annually in the United States alone.1 Although more prevalent in teenagers, acne can also have an impact on every age group.2 Both sexes and all races are prone to this often distressing skin condition.3 Many physicians trivialize this condition, .but considerable research has explored the psychosocial impact and quality of life for patients with acne. A study by Mallon et al4 assessed the quality of life for patients and compared it with other chronic conditions. They found that patients with acne have social, psychological, and emotional problems of the same magnitude as those with chronic asthma, epilepsy, diabetes, back pain, or arthritis. Acne contributes to low self-esteem and self-confidence and often leads to anger, frustration, and social withdrawal.5 A key factor in the pathogenesis of inflammatory acne is the proliferation of Propionibacteiium acnes.6-8 During adolescence, hormonal changes cause increased activity of the sebaceous glands.9,10 This results in excess sebum production and abnormal keratinization in the sebaceous gland duct leading to the follicle.11-12 Obstruction of the follicle then occurs, providing a favorable environment for P acnes proliferation.7,13 Additionally, excess sebum serves as an excellent nutritional source for P acnes populations.14 This process leads to inflammation and the resultant papules and pustules observed in patients with inflammatory acne.13 Many treatment options for acne currently exist, and a significant amount of money is spent by Americans each year on prescription and nonprescription
medications.15-16 These treatment options, however, have limitations. Topical acne preparations, such as benzoyl peroxide and retinoids, often irritate the skin and lead to decreased patient compliance.17-18 The mainstay treatment for mild to moderate acne is topical and/or oral antibiotics, but in recent years antibiotic resistance has become a major concern.19-22 In addition/oral antibiotic therapy is not effective for many patients, and a new therapeutic modality is needed for their acne. Topical retinoids often are selected for their effect on keratinization and anti-inflammatory properties.19-21 But these agents commonly cause a great deal of skin irritation, such as peeling, dryness, erythema, stinging, burning, and photosensitivity, precluding their use for many patients.23-25 Isotretinoin is an effective treatment for acne; however, it has numerous side effects and is reserved for severe or refractory cases.26-27 Chemical peels, laser ablation, and surgery generally are not directed to combat active disease but rather to mitigate the resultant scars and hyperpigmentation.28-29 That is why an effective, safe, nonirritating, and easy-to-use modality is highly desirable and needed.This study examined the Charme™ system, a hand-held device that electrolyzes a water-based sodium chloride solution and delivers etectrolyzed oxidized water (EOW) to the skin. The system produces fine particles, 40 urn or less in diameter, that are able to penetrate the epidermis and upper dermis. EOW has been used for many years in Japan for its antimicrobial properties in the treatment of thermal injury, pressure ulcers, and nosocomial infections.30-32 In addition, EOW has been used for cleaning and disinfecting medical equipment, such has hemodialysis units.33-35 Studies show that the device is active against a wide range of bacteria, including anaerobes, gram-positive and gram-negative species, and even viruses, including HIV and hepatitis 33,36 (Table). Furthermore, the device uses a naturally occurring substance, eliminating the need for artificial substances.
By producing an electrolyzed, low pH solution that is active against a wide variety of bacteria and delivering it to the skin, the device offers a potential treatment modality for mild to moderate acne. The purpose of this open-label pilot study was to determine the efficacy and tolerability of the device in the treatment of mild to moderate acne.
METHODS
A total of 31 subjects qualified and were enrolled in this split-face, open-label, clinical study. To be included, patients had to present with 5 to 50 facial noninflammatory lesions (open and closed comedones), 5 to 60 inflammatory lesions (papules and pustules), and no more than 3 cystic lesions on each side of the face. No other dermatologic disease could be present on the face. Patients were excluded from the study if they used topical acne medications in the preceding 14 days, systemic antibiotics in the past 30 days, or systemic retinoids in the last 6 months prior to initiation of treatment. Pregnant and lactating women, as well as women using oral contraceptives, were excluded from the study. Patients were not allowed to use any over-the-counter acne medications or washes during the study period. Only mild moisturizers and sunscreens were permitted throughout the study. Informed consent was obtained from all patients. For minors, a parent or legal guardian cosigned the informed consent and accompanied the minor patient on each visit during the treatment period.At baseline, each patient in the study gave a medical history and had a physical examination. A thorough assessment of the severity of acne was made, and the number and type of acne lesions were noted. Patients were given detailed information on how to use the EOW study device. This included demonstrations on how to pour the base solution (a low concentration of 2500 ppm sodium chloride) into the device. The evaluator also gave demonstrations on the distance (approximately 10 cm from the face) and duration (a minimum of 10 seconds to a maximum of 30 seconds) for using the device. Each patient was given an EOW device, 3 bottles of base solution, and 2 unscented, mild soap bars. At each visit, empty solution bottles were retrieved and recorded.
Each patient was instructed to use only the unscented, mild soap to cleanse the entire face twice a day. After the face was washed and dried, the EOW device was to be used only on the right side of the face for 10 to 30 seconds continuously twice daily (morning and night), while the left side of the face remained untreated (control). A log sheet was provided to each patient to keep track of the time, date, and duration of each treatment and any adverse effects experienced.
Efficacy and cutaneous tolerance were assessed 4 times over the trial period: at baseline and weeks 2, 4, and 8. At each of these visits, the number and type of acne lesions were evaluated, and patient and physician assessments were recorded. Vital signs and adverse effects also were recorded at each visit, and device compliance was reviewed. Telephone calls were made to each patient at 2-week intervals to stress device compliance and to assess the general well-being of the patient.
Cutaneous tolerance was assessed by determining the degree of erythema, peeling, stinging/burning, and pruritus. All cutaneous tolerance evaluations were graded on a 0 to 3 scale: 0=none; 1 =mild; 2=moderate; and 3=severe. Global improvement compared with baseline was graded by the physician at each follow-up visit, using this 6-point scale: 0=no signs of acne; 1=markedly (75) improved; 2=moderately (50) improved; 3=slightly (25) improved; 4=no improvement; and 5=worse. Using the same scale, patients graded improvement in their acne over the course of the treatment period. At the conclusion of the study, patient satisfaction with the EOW device was measured using a 0 to 3 scale: 0=not satisfied; 1=fair; 2=good; and 3=excellent. This patient questionnaire also included assessment of the EOW device, compared with conventional acne treatments, based on a numerical scale. Patients were photographed by a standardized method, using high-resolution digital photography at baseline and at each subsequent visit. Results of treatment versus baseline scores were assessed using a Hotelling T test and repeated-measures analysis of variance. All statistical tests were 2-sided, and values of .04 or less were considered statistically significant.
RESULTS
Patients
A total of 25 of 31 patients completed the 8-week efficacy and tolerability study; 2 patients were disqualified for treating the entire face with the EOW device, and 4 were lost to follow-up or moved to another geographic region. The mean age was 24.7 years, with a range of 15 to 47 years. The ethnicity groups represented in this study were: white (32), Asian (32), Hispanic (20), and African American
(16).Clinical Assessment
There were no significant differences between the right and left side of the face at baseline in any of the parameters to be evaluated. The mean reduction in the total number of acne lesions from baseline to the end of treatment (8 weeks) was 40.9 for the EOW-treated side (right side) and 18 for the untreated side (left side) (P=.04). There was a consistent reduction in total lesion count in the EOW-treated side, with clinically relevant reductions at week 8, compared with an inconsistent clinical response in the untreated side (Figure 2).
Figure 2. Patient is a 28-year old Caucasian female with long-standing inflammatory acne refractory to oral and topical antibiotics, topical retinoids and benzoyl peroxide.
Treated Side of Face
2A. Before treatment, numerous inflammatory acne lesions are noted. (Right side of face)2B. After 8 weeks of treatment with CHARME twice daily, significant improvement is noted with minimal irritation.
(Right side of face)Untreated side of face
2C. Same patient at baseline without treatment on left side of face.2D. Control at 8 weeks. No improvement observed in acne lesion counts.
The mean reduction in the number of inflammatory lesions (pustules and papules) from baseline to the end of treatment was 49.1 in the EOW-treated side, compared with 9.9 in the untreated side
(P<.01). The mean reduction in each specific type of inflammatory lesion was also clinically
significant. The treated side had a mean reduction of 75.7 and 42.0 in the number of pustules and papules, respectively. The untreated side showed only a mean reduction of 19.4 and 7.38 in the number of pustules and papules, respectively (P=.002) (Figures 3 and 4).There was not a statistically significant reduction in the number of noninflammatory acne lesions (open/closed comedones) or cysts between the treated and untreated side of the face (P=.336).
Physician-assessed global improvement increased steadily, as noted in the percentage of patients improving from baseline, with 100 of patients showing 25 or more improvement by week 8 (Figure 5). Patient-assessed global improvement in acne also steadily increased during the course of the study period. At week 8, 50 of the patients graded overall their improvement as moderate, with improvement of 50 or more for the right side of the face (Figure 6). .In addition, 75 of the patients in the study indicated that they preferred the EOW delivery system over conventional topical acne medications.
Safety Assessment
The EOW system was well tolerated by an overwhelming majority of patients in the study. No patients stopped or temporarily ceased treatment due to side effects. One patient had transient pruritus, and 2 others had mild erythema during the first 2 days of treatment. Two additional patients experienced burning/stinging and treatment-associated peeling. All cutaneous side effects resolved by the week 2 visit (Figure 7).Patient Satisfaction Assessment
In their assessments, 80 of the patients in the study rated their satisfaction with the EOW device as good or excellent, while 16 rated the study device as fair. Only 4 were dissatisfied with the EOW device (Figure 8).COMMENT
EOW is produced with an anode current by electrolyzing salt-containing water through a
diaphragm.37 EOW which has a high positive oxidation-reduction potent (ORP) and high concentrations of dissolved chloride and oxygen, functions as a bactericide and is used clinically for the treatment of various types of infection and for cleaning and disinfecting medical equipment.37,39 Since the 1990s, electrolyzed sodium chloride solutions, which contain high free-chloride concentrations, have been investigated for various clinical applications in Japan. Currently, 2 types of electrolyzed solutions are available: electrolyzed weak acid water (EWW) and electrolyzed strong acid water (ESW). The study device generated ESW by electrolysis of an NaCI solution, using positive and negative electrodes in compartments separated by a cationic membrane; ESW was obtained from the well of the positive electrode (Figure 9).37Electrolysis Process
Figure 9. The process of electrolysis (steps 1-5): After the water-based, sodium chloride solution (base solution) is Introduced into the electrolyzed oxidized water (EOW) device, the electrolysis process begins. Within seconds, EOW is emitted as a fine mist to the skin. Nontoxic alkaline solution is collected in the drainage tank and emptied after use.
This process can be summarized in the following chemical formula.37-39
Positive electrode:
H20 ? ½O2 + 2H+ + 2e-
2Cl- ? Cl2 + 2e-
CI2 + H2O ? H+ + Cl- + HClO
Negative electrode:
2H2O + 2e- ? H2 + 2OH-The water at the positive electrode becomes EOW, which has a low pH and a high ORP and contains high concentrations of dissolved chloride, oxygen, and Hydroxyl radicals. Water at the negative electrode becomes a basic aqueous solution that has a high pH and a low ORP and high concentrations of alkaline minerals.
All microorganisms require a certain mitochondrial environment for survival; a pH in the range of 2 to 12 and ORP from -400 to +850 mV are needed to survive and proliferate.40 In this study, the EOW device produced a solution that has a pH in the range of 2.3 to 3.0 and a high ORP in the range of +1100 to +1300 mV.37 It also generated high concentrations of hypochlorous acid, dissolved oxygen (9-15 ppm), and hydroxyl radicals.37 This was the solution that was emitted in a fine mist by the EOW device after the electrolysis process.
The EOW produced by the study device falls outside the required parameters for the mitochondrial function of microorganisms. This served as the basis for the proposed mechanism of action against the P acnes population. Additionally, the high concentrations of hypochlorous acid and Hydroxyl radicals produced by the treatment solution are well documented antimicrobial agents that markedly increase in low pH solution. Moreover, a study by Shimizu et al41 reported that almost all organisms will perish within 10 seconds in EOW because of the synergistic activity of the resultant
radicals (eg, Cl-, hydroxyl radical, hydrogen peroxide) generated in EOW.42 These radicals react with oxygen and destroy P acnes by damaging its cell lipid membrane, denaturing proteins, and preventing replication by severing its DNA.43 Because EOW does not provide a long-lasting antibacterial effect after application, repeated applications are necessary.37,44-46 In this study, patients were instructed, to use the EOW device twice daily for at least 10 seconds.To further analyze the bactericidal effect of EOW research studies on inhibiting Pseudomonas aeruginosa growth by EOW can be used as a model. Using electron microscopy, P aerugi'nosa growth was completely inhibited by EOW. Morphological changes noted on electron microscopy showed that EOW induced breaks and blebs in the outer membrane cell wall (average diameter of bleb was 28 nm), which were not seen in unelectrolyzed sodium chloride solution.36 To assess whether or not the effect of EOW penetrated deep into the bacterial cells, the presence of chromosomal DNA was detected by restriction fragment length polymorphism, or RFLP, assay.36 No bands were detected using EOW, while bacterial samples treated with unelectrolyzed sodium chloride revealed strong band formation.36
The study showed that the EOW treatment, administered twice daily for 8 weeks in patients with mild to moderate acne, resulted in an overall reduction of 40.9% in mean acne lesion counts. A similar reduction was noted in the mean inflammatory acne lesion counts measured independently (49.1%). A greater decrease was observed when measuring individual inflammatorylesions—75.7% for pustules and 42.0% for papules.
The mechanism by which the study treatment improved inflammatory acne lesions is most likely the EOW. The EOW is antimicrobial and decreases P acnes colony populations. This proposed mechanism of action is supported by the observation of the authors that pustules, followed by papules, had the most significant reductions.
The study demonstrated that the EOW system is potentially effective on inflammatory lesions but is not an effective comedolytic agent. To treat patients with inflammatory acne, the authors suggest oral and topical
antibiotics can be eliminated by using a strong comedolytic agent, such as a topical retinoid, in combination with the EOW treatment. Moreover, the EOW device, working in conjunction with topical retinoids, could possibly decrease common cutaneous side effects associated with topical retinoids.Patient satisfaction with the EOW treatment was rated very high, with 80% of the patients in the study assessing satisfaction with the study device as good or.excellent. Only 4% of the patients were dissatisfied with the device. Moreover, 50% of the patients reported a moderate improvement at week 8. Of particular note, 75% of the patients indicated that they would rather use an EOW delivery system than conventional forms of treatment.
In contrast to the likelihood of side effects from other anti acne products, such as topical retinoids and benzoyl peroxide, the EOW treatment and its delivery system did not produce significant clinical signs or subjective symptoms of skin irritation, stinging/burning, erythema, pruritus, or peeling. In fact, 96% of the patients did not experience any burning/stinging or peeling. Only 4% experienced pruritus, and 8% experienced treatment-associated erythema. All side effects experienced by patients during the treatment period were completely resolved by the week 2 visit.
From a pharmacoeconomic perspective, the EOW system appears to be cost-effective, though it is difficult to conduct a cost-effectiveness analysis comparing the EOW treatment to current acne therapies because of the multitude of factors to consider in assessing acne treatment costs. When evaluating the estimated costs of various acne therapies in 2001, however, the study device afforded the patient with a more cost-effective method in treating acne.16-46 For instance, 40% of patients in 2001 spent an average of $293.03 on acne treatments, and 20% of patients spent an average of $813.24 per year on acne medications.16-46 Tolerability of these medications can also influence the overall cost because more office visits are required, and alternative medications may be prescribed to increase compliance.
Clinical studies have repeatedly shown that combination therapy yields the best results when treating acne.19-21, 47. A common approach is to use topical or systemic antibiotics with topical retinoids.19-21 Topical and oral antibiotics, however, often are associated with the emergence of resistant bacteria and adverse effects, such as gram-negative folliculitis, vaginal candidiasis, discoloration of teeth, headaches, depression, gastrointestinal upset, and dose-related phototoxic effects.48-55 Other antibiotic options, such as trimethoprim and sulfamethoxazole, clindamycin, and ciprofloxacin are effective but are limited by toxicities.56 The study suggested that EOW treatment is effective in combating inflammatory acne and is a possible alternative to oral/topical antibiotics in the management of this condition. Oral and topical antibiotics could be used as a second-line therapy, an option that would most likely help reduce the resistance of P acnes and antibiotic-associated toxicities.
CONCLUSION
The EOW device and its delivery system to the skin is an alternative modality for treating acne. Although it has never been studied specifically for acne, scientific evidence from other applications makes this device a practical and logical consideration. Used for many years in Japan, EOW has been shown to be effective against many different types of bacteria, viruses, and fungi. This study showed that the EOW device can reduce inflammatory acne lesions, namely papules and pustules.Patient assessments indicated satisfaction with and acceptance of the EOW system, which may be attributed to its ease of use and relatively few of the side effects commonly experienced with topical acne regimens. In addition, a possible cost savings makes this device an even more attractive option for patients. Other advantages of EOW treatment include: a relatively short duration of therapy (8 weeks), good patient compliance, and lack of antibiotic resistance. Although this is the first study illustrating the efficacy and tolerability of the EOW device in treating acne, the results and patient satisfaction seem promising. Future studies are needed to see if EOW has additional mechanisms of action in treating inflammatory acne.
Acknowledgment—The authors extend special thanks to Gary M. Guglechuk for assistance in statistical analysis and to Vivian Tarn for assistance with the graphics.
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